The Laboratory of Biomechanics and Biomedical Engineering of the University of Patras has more than 25 years of experience in the fields of biomechanics and biomaterials emphasizing in the domain of cell-material interactions.
Blood compatibility testing using static and dynamic blood-material contact, cell-materila interactions in the case of diffenrent cell cultures, mechanical properties of cells, tissues and biomaterials are some of the main research interests of our group.
More recently nanotechnology and nanoidentation techniques, functionalization of polymeric surfaces via selected biomolecules immobilization techniques as well as techniques to study endothelial cell culture on biomaterials under dynamic (flow, mechanical stress) conditions have been developed.
Teaching
CHEMISTRY (1st year of studies)
BIOMECHANICS (section of applied mechanics and biomechanics): studying the mechanics of living organisms
BIOMATERIALS (section of apllied mechanics and biomechanics): studying materials that come from living organisms and materials that can work in living organisms
ARTIFICIAL ORGANS (section of applied mechanics and biomechanics): studying the design of artificial systems that can substitute totally or partially the function of the living organism systems
Research
Recent research projects:
2004-2008: COST Action 537, EEC,: Core Laboratories for the Improvement of Medical Devices in Clinical Practice from the Failure of the Explanted Prostheses Analysis (FEPA)
2005-2007: Project Pythagoras II “Expression and role of proteoglycans in the differentiation of osteoblastic progenetor cells”
2005-2008: Project STREP VASCUPLUG of the EEC: “Bioreactive Composite Scaffold Design for Improved Vascular Connexion of Tissue Engineered Products”
2006-2008: Project INTERREG between Greece and Italy: “Applied Biomaterials”
2006-2009: Project Karatheodoris: Biomechanics and biocompatibility of biomaterials applied in histotechnology.
2009-2013: FP7 Nanoparticles for Therapy and Diagnosis of Alzheimer Disease
2010-2011 : Project IKYDA 2010Greece-Germany: “Histotechnology of Biomaterials”
2010-2011: Project IKYDA 2010Greece-Germany:”Combination of biomimetic surface coatings and mechanical forces to control the differentiation of human embryonic cells”
2010-2013: Project Synergasia :”Nano-arthro-chondros”
2010-2013: Project Synergasia :”BIOMINY”
2012-2014: Project LEADERA: e-iliza-Development of an e-health system in orthopedics
2012-2016: Project FP7-people-ITN/Marie Curie “TECAS” tissue engineering of vardiovascular implants.
Selected Publications:
M. Katsikogianni and Y.F. Missirlis, “Concise review of mechanisms of bacterial adhesion to biomaterials and of techniques used in estimating bacteria-material interactions”, European Cells & Materials Journal,8, 37-57,2004
G. Koromila, GPA Michanetzis, YF Missirlis and SG Antimisiaris. Heparin incorporating liposomes as a delivery system of heparin from PET-covered metallic stents: Effect on haemocompatibility. Biomaterials, Volume 27, I12, 2006, p. 2525-2533
M. Itskov, A. E. Ehret, and D. Mavrilas. “A polyconvex anisotropic strain energy function for soft biological tissues” Biomech Model Mechanbiol 5; 17-26 (2006).
D.. Mavrilas, P.G. Koutsoukos, E.N. Koletsis, E. Apostolakis, D. Dougenis. “In-vitro evaluation for potential calcification of biomaterials used for staple line reinforcement in lung surgery ” Experimental Biology & Medicine 231; 1712-1717 (2006).
Katsikogianni M. G., Syndrevelis C. S., Amanatides E., Mataras D. S., Missirlis Y. F., “Plasma treated and a-C:H coated PET performance in inhibiting bacterial adhesion”, Plasma Processes and Polymers, 4( S1), : S1046-S1051 ,2007
Th. S. Tsapikouni and Y.F.Missirlis, “pH and ionic strength effect on single fibrinogen molecule adsorption on mica studied with AFM”, Colloids and Surfaces B: Biointerfaces, 57, (1) : 89-96, 2007
Moutzouri A., Skoutelis A., Gogos H., Missirlis Y., Athanassiou G. ” Red blood cell deformability in patients with sepsis: A marker for prognosis and monitoring of severity” Clinical Hemorheology and Microcirculation, 36 (2007) 291-299.
DD Deligianni and CA Apostolopoulos. Multilevel finite element modeling for the prediction of local cellular deformation in bone. Biomech ModelMechanobiol 7(2), 2008, 151-9. Epub 2007 Apr 13.
GPAK Michanetzis, YF Missirlis and SG Antimisiaris. Haemocompatibility of nanosized drug delivery systems: Has it been adequately concidered? Journal of Biomedical Nanotechnology, V4, 2008, p 218-233.
Moutzouri A., Athanassiou G., Gogos C., “Red blood cells deformability from patients with Diabettes” Journal Infection Feb-2008.
CA Apostolopoulos and DD Deligianni. Prediction of local cellular deformation in bone-Influence of microstructure dimensions. Journal of Neuronal and Musculoskeletal Interactions Interactions 9(2), 2009, 99-108.
M. Krings, D. Kanellopoulou, P.G. Koutsoukos, D. Mavrilas, B. Glasmacher: “Development of a new combined test set-up for accelerated dynamic pH controlled in vitro calcification of porcine heart valves”. Int. J for Artificial Organs 32(11); 794-801(2009).
P Kokkinos, I. Zarkadis, T. Panidia and D. Deligianni. Estimation of hydrodynamic shear stresses developed on human osteoblasts cultured on Ti-6Al-4V and strained by four point bending. Effects of mechanical loading to specific gene expression. Journal of Materials Science: Materials in Medicine 20, 2009, 655-65.
Stergios Dermenoudis and Yannis Missirlis, “Design of a novel rotating wall bioreactor for the in vitro simulation of the mechanical environment of the endothelial function “, J.Biomechanics, 43(7):1426-1431,2010
Th. S. Tsapikouni and Y.F.Missirlis, “Measuring the force of single protein molecule detachment from surfaces with AFM”, Colloids and Surfaces B: Biointerfaces, 75(1): 252-259,2010.
M.G. Katsikogianni, Y.F. Missirlis, “Bacterial adhesion onto materials with specific surface chemistries under flow conditions”, J. of Materials Science: Materials in Medicine , 21:963-968, 2010.
Kaleridis V., Athanassiou G., Deligianni D., Missirlis Y. “Effects of nucleus in leukocyte deformability at slow deformation rates” Clinical Hemorheology 45(1) 53-65, 2010 .
S.I. Gkizas, E. Apostolakis, E. Pagoulatou, D. Mavrilas, D.J. Papachristou, E. Koletsis, A. Papalois, H. Papadaki, D. Alexopoulos “Aldosterone receptor inhibition alters the viscoelastic biomechanical behavior of the aortic wall” Experimental Biology & Medicine 235;311-316 (2010).
S. Dermenoudis and Y.F.Missirlis, ” Bioreactors in Tissue Engineering”. Invited Review in Advanced Biomaterials, 2010.
A.Soininen, J.Levon, M.Katsikogianni, K.Myllymaa, R .Lappalainen, Y.T.Konttinen, T.J.Kinnari, V.-M.Tiainen,Y.Missirlis, ” In vitro adhesion of staphylococci to diamond-like carbon polymer hybrids under dynamic flow conditions” J Mater Sci: Mater Med, 22(3): 629-636, 2011
Th.Tsapikouni and Y.F.Missirlis, “P-selectin /ligand unbinding force measured with Atomic Force Microscopy: Comparison of two chemical protocols for the tethering of single molecules”, Journal of Molecular Recognition, 24(5): 847-853, 2011.
E. Pagoulatou, I.E.Triantaphyllidou, D.H. Vynios, D.J. Papachristou, E. Koletsis D.Deligianni, D. Mavrilas. Biomechanics and structural changes following the decellularization of bovine pericardial tissues for use as a tissue engineering scaffold”.
J Mater Sci: Mater Med, 23(6);1387-96 (2012).
PA. Kokkinos, R Wright, PB. Kirby, AND DD. Deligianni, Differential Regulation of Osteoblasts by Microstructural Features of Titanium Substrata. Trends Biomater. Artif. Organs 26(1), 2012, 16-24.
PA. Kokkinos, PG Koutsoukos, and DD Deligianni, Detachment strength of human osteoblasts cultured on hydroxyapatite with various surface roughness. Contribution of integrin subunits. J Mater Sci Mater Med, 23(6) (2012), 1489-1498.
Patents:
European Patent Office:
Membranes made from P(AN/NVP)-copolymers with both haemo and tissue compatibility and their application in medical field.
Inventors: GKSS (Berlin): Albrect, Groth, Malsh, Seifert, University of Patras: Michanetzis, Missirlis, Free University of Berlin: Fey-Lamprecht, Gross, Strathclyde University: Courtney, Grant, Holland Biomaterials Group: Engbers
Greek Industrial Property Organization:
Description of a method to attach liposomic forms of heparin and/or other biomolecules on polymeric surfaces in order to control the release of their active substance(s) Inventors: Missirlis I, Antimisiaris S, Koromila G and Michanetzis G.
Equipment
Equipment facilities include
Atomic Force Microscope (Veeco) / Nanoindemtation (Hysitron) system, Scanning Electron Microscope, Confocal / Epifluorescent Microscope (Nikon) (3 channels+transmission), clean room / cell culture facilites, mechanical testing devices, PCR, centrifuges, deep freezer, plate readers, …